細胞側視系統
細胞側視系統為 Dr.
Chi-Hung Lin 和 Szecheng Lo 的實驗室所研發，此系統可即時且高空間解析度的觀察位於培養基上的細胞生長。
利用此技術，你可以很簡單的用傳統的光學顯微鏡來觀察培養中細胞的側面。並且可與所有的顯微鏡觀測技術做結合，包含了暗視野觀測，亮視野觀測，相位差觀測，微分干涉( differential interference contrast)，以及螢光顯微鏡。相較於降低時間解析度的共厄焦顯微鏡，細胞側視技術可改善此缺點使細胞側面及時成像。
Owing
to the special optical configuration of the Side View Technology,
the spatial resolution can also tuned up to optical limits,
without the limitations of the mechanical components, such
as stepper motors. Consequently many fast and subtle biological
phenomena can be resolved. Moreover, cells or tissues at the
side view configuration could also be manipulated by optical
tweezers simultaneously. This totally novel combination
of observing and manipulating cells may open a new window
of biomedical research.
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Cell
spreading observed by the Side View Technology. Two
Chinese Hamster Ovary (CHO) cells expressing integrin
aIIbb3
were put on a substrate coated with rhodostomin,
and the process of cell spreading was observed using
the Side View Technology with time-lapse recording.
The cells were shown to spread and flatten within about
1.5 hours. Similar technique was successfully applied
to platelets to observe their
cellular activities and spreading.
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Cell
membrane glycoproteins decorated by a fluorescein-conjugated
lectin, concanavalin A (Con A) observed by the Side
View Technology. Con A recognizes a-linked
mannose, which commonly occurs in glycoproteins. As
shown here, the Side View Technology is capable of observing
cells with fluorescent microscopy.
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The
combination of side view microscopy and optical
tweezers. A bead coated with poly-L-lysine (arrow)
was trapped by optical tweezers
and put on the surface of a Chinese Hamster Ovary (CHO)
cell. The entire process was observed via the Side View
Technology. This combined technology can be used in
the observation of many cellular activities such as
endocytosis and intracellular signaling.
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Links:
Updated
6/13/2013. Copyright© 2001
Jin-Wu Tsai. All rights reserved.
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